4EI7
Crystal structure of Bacillus cereus TubZ, GDP-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2011-06-28 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.826, 76.015, 96.931 |
| Unit cell angles | 90.00, 104.59, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.185 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.059 | 0.027 | 0.464 |
| Number of reflections | 52862 | ||
| <I/σ(I)> | 11.1 | ||
| Completeness [%] | 97.8 | 96.1 | 96.9 |
| Redundancy | 5.2 | 5.1 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 298 | 50% PEG 400, 0.1M MES, 1mM GTPgammaS, pH 5.5, VAPOR DIFFUSION, temperature 298K |
