4EHM

RabGGTase in complex with covalently bound Psoromic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-06-23
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	66.421, 91.078, 114.725
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.400 - 2.200
R-factor	0.18875
Rwork	0.186
R-free	0.25148
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.409
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASES
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.400
High resolution limit [Å]	2.200
Rmerge	0.045
Number of reflections	35947
<I/σ(I)>	25.7
Completeness [%]	99.7
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	285	20mM Hepes pH7.2, 100mM NaCl, 2mM DTT, 15% PEG 3350, 0.2M Ca Acetate, 0.1M Hepes pH7.2, VAPOR DIFFUSION, HANGING DROP, temperature 285K