4EGT
Crystal structure of major capsid protein P domain from rabbit hemorrhagic disease virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-21 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.99985 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.930, 88.144, 135.599 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.950 - 2.000 |
| R-factor | 0.20073 |
| Rwork | 0.199 |
| R-free | 0.23154 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gh8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.245 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.089 | 0.231 |
| Number of reflections | 35882 | |
| <I/σ(I)> | 29.8 | 8.3 |
| Completeness [%] | 99.1 | 99.9 |
| Redundancy | 3.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 289 | 0.1 M sodium acetate, 1.1 M succinic acid, 1.0% PEG2000 MME, pH 5.5, VAPOR DIFFUSION, temperature 289K |
