4EGK
Human Hsp90-alpha ATPase domain bound to Radicicol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-20 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.440, 43.880, 53.640 |
| Unit cell angles | 90.00, 113.81, 90.00 |
Refinement procedure
| Resolution | 32.710 - 1.690 |
| R-factor | 0.20343 |
| Rwork | 0.201 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.972 |
| Data reduction software | PROTEUM2 (suite) |
| Data scaling software | PROTEUM2 (suite) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.710 |
| High resolution limit [Å] | 1.690 |
| Rmerge | 0.037 |
| Number of reflections | 25007 |
| Redundancy | 3.75 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 273 | 0.2M MgCl2, 0.1M HEPES, 20%w/v PEG2000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
