4EG9

1.9 Angstrom resolution crystal structure of Se-methionine hypothetical protein SAOUHSC_02783 from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Detector technology	CCD
Collection date	2012-03-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	43.346, 60.707, 94.156
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.890 - 1.900
R-factor	0.21801
Rwork	0.215
R-free	0.27143
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.364
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.084	0.438
Number of reflections	19583
<I/σ(I)>	16.4	3.1
Completeness [%]	96.3	80.3
Redundancy	4.3	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	7.0 mg/mL protein in 0.25 M sodium chloride, 0.01 M Tris, pH 8.3, screen: TRAP, H1, 0.1 M HEPES, pH 7.0, 30% v/v Jeffamine, VAPOR DIFFUSION, SITTING DROP, temperature 295K