4EFB
Crystal structure of DNA ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.130, 86.010, 56.840 |
| Unit cell angles | 90.00, 100.87, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.200 |
| R-factor | 0.1956 |
| Rwork | 0.196 |
| R-free | 0.27710 |
| Structure solution method | FOURIER SYNTHESIS |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.064 |
| Number of reflections | 17616 |
| Completeness [%] | 80.3 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.6M Ammonium sulfate, 0.1M Na Cacodelate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
