4EF2

Crystal structure of a pheromone cOB1 precursor/lipoprotein, YaeC family (EF2496) from Enterococcus faecalis V583 at 2.10 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91837,0.97966,0.97901
Spacegroup name	P 21 21 21
Unit cell lengths	46.225, 75.691, 143.815
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.763 - 2.100
R-factor	0.1898
Rwork	0.188
R-free	0.23220
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.858
Data reduction software	XDS
Data scaling software	XSCALE (December 29, 2011)
Phasing software	SHELX
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.763		2.170
High resolution limit [Å]	2.100	4.510	2.100
Rmerge	0.040	0.017	0.746
Number of reflections	28845	5557	3484
<I/σ(I)>	12.37	37.1	1.4
Completeness [%]	90.6	95.4	64.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	30.0% polyethylene glycol 400, 0.1M CHES pH 9.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K