4EEW
Crystal structure of the Ubl domain of BAG6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-18 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9767 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.572, 43.193, 51.734 |
| Unit cell angles | 90.00, 104.33, 90.00 |
Refinement procedure
| Resolution | 32.720 - 1.300 |
| R-factor | 0.18527 |
| Rwork | 0.184 |
| R-free | 0.19975 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.185 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.720 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 28619 | |
| <I/σ(I)> | 25.9 | 3 |
| Completeness [%] | 93.9 | 58.6 |
| Redundancy | 2.3 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 0.2 M trimethylamine N-oxide, 0.1 M Tris, pH 8.5, 20% w/v PEG2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
