4EEU

Crystal structure of phiLOV2.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Detector technology	CCD
Collection date	2010-11-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.11611
Spacegroup name	P 43 21 2
Unit cell lengths	40.967, 40.967, 130.554
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.478 - 1.407
R-factor	0.2018
Rwork	0.201
R-free	0.21230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ees
RMSD bond length	0.009
RMSD bond angle	1.234
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall
Low resolution limit [Å]	26.500
High resolution limit [Å]	1.400
Number of reflections	21959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	298	25% MPEG 2K, 0.2 M imidazole malate, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K