4EEQ
Crystal structure of E. faecalis DNA ligase with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.380, 86.460, 57.000 |
| Unit cell angles | 90.00, 101.05, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.100 |
| R-factor | 0.1919 |
| Rwork | 0.184 |
| R-free | 0.24470 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.779 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.067 |
| Number of reflections | 25305 |
| Completeness [%] | 81.0 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.6M Ammonium sulfate, 0.1M Na cacodelate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
