4EEB
CorA coiled-coil mutant under Mg2+ absence
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-27 |
| Detector | MARMOSAIC CCD300 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 125.600, 221.900, 106.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 3.800 |
| R-factor | 0.3009 |
| Rwork | 0.299 |
| R-free | 0.34340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iub |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.336 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.920 | 3.900 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Number of reflections | 24030 | |
| <I/σ(I)> | 3.3 | |
| Completeness [%] | 80.4 | 34.1 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | Jeffamine M-600, CsCl, pH 6.5, temperature 277K, VAPOR DIFFUSION, HANGING DROP |
