4EE1

The structure of the S. aureus DnaG RNA Polymerase Domain bound to CTP and Manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.115
Spacegroup name	P 61
Unit cell lengths	151.986, 151.986, 38.826
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.749 - 2.020
R-factor	0.1838
Rwork	0.182
R-free	0.21710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.698
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	PHENIX (AutoMR)
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.749	49.745	2.130
High resolution limit [Å]	2.020	6.390	2.020
Rmerge		0.043	0.682
Number of reflections	32386
<I/σ(I)>	14.8	14.4	1.1
Completeness [%]	94.7	99.6	100
Redundancy	11.4	10.8	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	0.15M sodium thiocyanate, 0.1M Bis-Tris, 13% PEG3350, 2% Benzamidine, 2.5 mM CTP, 5 mM MnCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K