4EDV

The structure of the S. aureus DnaG RNA Polymerase Domain bound to pppGpp and Manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.115
Spacegroup name	P 61
Unit cell lengths	151.334, 151.334, 38.691
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.108 - 2.010
R-factor	0.1686
Rwork	0.167
R-free	0.20320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.720
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX (AutoMR)
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	131.059	2.120
High resolution limit [Å]	2.010	2.010
Rmerge	0.083	0.572
Number of reflections	34211
<I/σ(I)>	17.8	1.3
Completeness [%]	99.8	99.8
Redundancy	8.1	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	0.15M sodium thiocyanate, 0.1M Bis-Tris, 13% PEG3350, 2% Benzamidine, 2.5 mM pppGpp, 10 mM MnCl2, 1 mM MgCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K