4EDU

The MBT repeats of human SCML2 in a complex with histone H2A peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-26
Detector	ADSC Q315
Wavelength(s)	0.97937
Spacegroup name	P 43 21 2
Unit cell lengths	69.949, 69.949, 169.609
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.580
R-factor	0.206
Rwork	0.204
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oi1
RMSD bond length	0.015
RMSD bond angle	1.463
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.970	43.970	2.720
High resolution limit [Å]	2.580	8.160	2.580
Rmerge	0.111	0.030	0.980
Rmeas		0.030	1.050
Total number of observations		3348	15670
Number of reflections	15321
<I/σ(I)>	15.5034
Completeness [%]	99.9	98.95	99.95
Redundancy	7.71	6.31	7.92

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9.5	291	1.6M NH4PO4, 0.1M Tris/HCl, 1:5 molar ratio of protein:peptide, protein concentration 35 mg/ml, pH 9.5, vapor diffusion, hanging drop, temperature 291K