4EDG

The structure of the S. aureus DnaG RNA Polymerase Domain bound to ATP and Manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.115
Spacegroup name	P 61
Unit cell lengths	151.261, 151.261, 38.665
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.665 - 2.003
R-factor	0.1633
Rwork	0.162
R-free	0.19690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4e2k
RMSD bond length	0.008
RMSD bond angle	1.062
Data reduction software	XDS
Data scaling software	SCALA (3.3.20)
Phasing software	PHENIX (AutoMR)
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	130.996	43.665	2.110
High resolution limit [Å]	2.003	6.330	2.000
Rmerge		0.023	0.287
Total number of observations		5971	26029
Number of reflections	34499
<I/σ(I)>	22.1	25	2.8
Completeness [%]	99.8	98.9	98.8
Redundancy	5.4	5.1	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	292	0.15M sodium thiocyanate, 0.1M Bis-Tris, 13% PEG3350, 2% Benzamidine, 2.5 mM ATP, 5 mM MnCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K