4ED9
Crystal structure of a CAIB/BAIF family protein from Brucella suis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-09 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.400, 62.540, 70.310 |
| Unit cell angles | 90.00, 117.81, 90.00 |
Refinement procedure
| Resolution | 62.190 - 1.950 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.21700 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.385 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 62.190 | 2.000 | |
| High resolution limit [Å] | 1.950 | 8.720 | 1.950 |
| Rmerge | 0.063 | 0.022 | 0.217 |
| Number of reflections | 25803 | 314 | 1785 |
| <I/σ(I)> | 26.86 | 70.6 | 6.2 |
| Completeness [%] | 98.0 | 96.9 | 92.3 |
| Redundancy | 8.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 290 | Internal tracking number 230921. JCSG Core Suite III well A4. 0.1M CHES Buffer pH 9.5, 30.0% w/v PEG3,000, 20% ethylene glycol. BrsuA.18159.a.A1 pw34608 40mg/ml., vapor diffusion, sitting drop, temperature 290K |
