4ED6

Human DNA polymerase eta - DNA ternary complex: Reaction in the AT crystal at pH 6.7 for 15 hr, Sideway translocation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	CCD
Collection date	2011-05-27
Detector	RIGAKU SATURN A200
Spacegroup name	P 61
Unit cell lengths	98.786, 98.786, 82.431
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.012 - 2.211
R-factor	0.2009
Rwork	0.198
R-free	0.25680
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.004
RMSD bond angle	0.818
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.260
High resolution limit [Å]	2.200	2.200
Number of reflections	20425
<I/σ(I)>	8.4	2.1
Completeness [%]	89.2	78.1
Redundancy	2.2	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	0.1M MES, 6-17%(W/V) PEG 2K-MME, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K