4ECH
Yeast Polyamine Oxidase FMS1, H67Q Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 161.160, 102.000, 75.830 |
Unit cell angles | 90.00, 93.12, 90.00 |
Refinement procedure
Resolution | 39.377 - 2.400 |
R-factor | 0.2182 |
Rwork | 0.216 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rsg |
RMSD bond length | 0.007 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.380 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 47765 | |
<I/σ(I)> | 15.7 | 2.7 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 3.7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 20% polyethylene glycol 3350, 0.2 M sodium acetate, 0.1 M bis-Tris propane, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |