4EBR

Crystal structure of Autophagic E2, Atg10

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97951, 0.97966, 0.96
Spacegroup name	P 61 2 2
Unit cell lengths	127.833, 127.833, 169.589
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.835 - 2.701
R-factor	0.224
Rwork	0.211
R-free	0.25110
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.418
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.835	2.750
High resolution limit [Å]	2.701	2.700
Number of reflections	23007
Completeness [%]	98.6	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	50mM bis-Tris-HCl (pH 6.15), 3M NaCl, 1mM 1,4-diacetoxymercury-2,3-dimetoxybutane, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8	293	50mM bis-Tris-HCl (pH 6.15), 3M NaCl, 1mM 1,4-diacetoxymercury-2,3-dimetoxybutane, VAPOR DIFFUSION, HANGING DROP, temperature 293K