4EB7
A. fulgidus IscS-IscU complex structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.469, 96.129, 152.793 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.370 - 2.750 |
| R-factor | 0.21665 |
| Rwork | 0.214 |
| R-free | 0.27284 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.641 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 81.370 |
| High resolution limit [Å] | 2.750 |
| Number of reflections | 29148 |
| <I/σ(I)> | 14.76 |
| Completeness [%] | 97.5 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | proteins 20 mg/mL (in buffer 13 mM Tris and 38 mM NaCl) mixed 1:1 with 0.2 M Ammonium acetate, HEPES 100 mM, PEG 3350 25%, pH 7.5, VAPOR DIFFUSION, HANGING DROP |
