4EAX

Mouse NGF in complex with Lyso-PS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.009
Spacegroup name	H 3
Unit cell lengths	96.529, 96.529, 163.780
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.500 - 2.300
R-factor	0.2208
Rwork	0.219
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1btg
RMSD bond length	0.010
RMSD bond angle	1.234
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.500	2.380
High resolution limit [Å]	2.300	2.300
Rmerge		0.493
Number of reflections	24969
<I/σ(I)>		2.7
Completeness [%]	98.7	89.3
Redundancy		7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	0.1M Imidazole, 1.0M Sodium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	0.1M Imidazole, 1.0M Sodium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K