4EAE
The crystal structure of a functionally unknown protein from Listeria monocytogenes EGD-e
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 61 |
| Unit cell lengths | 87.964, 87.964, 94.631 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.593 - 1.320 |
| R-factor | 0.1592 |
| Rwork | 0.158 |
| R-free | 0.17730 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.111 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 1.350 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Rmerge | 0.064 | 0.620 |
| Number of reflections | 97196 | |
| <I/σ(I)> | 43 | 3.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2.1M DL-malic acid., pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
