4E9O
Vaccinia D8L ectodomain structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.100002 |
| Spacegroup name | P 41 |
| Unit cell lengths | 71.281, 71.281, 44.415 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.900 - 1.420 |
| R-factor | 0.17434 |
| Rwork | 0.174 |
| R-free | 0.18987 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4etq |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.349 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.900 | 1.450 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Number of reflections | 40635 | |
| <I/σ(I)> | 33.6 | 2.7 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 0.2 M sodium iodide, 20% PEG3350, VAPOR DIFFUSION, temperature 298K |
