4E9L

FdeC, a Novel Broadly Conserved Escherichia coli Adhesin Eliciting Protection against Urinary Tract Infections

Replaces:  3TKV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.92
Spacegroup name	P 21 21 2
Unit cell lengths	44.630, 150.930, 47.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.380 - 1.900
R-factor	0.1794
Rwork	0.178
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb entries 1CWV 1f00
RMSD bond length	0.010
RMSD bond angle	1.170
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Rmerge	0.084
Number of reflections	24734
<I/σ(I)>	23.9
Completeness [%]	95.6
Redundancy	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	20% PEG-6000, 0.1M Bicine, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K