4E9H
structure of glycosylase domain of MBD4 bound to 5hmU containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | .9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.090, 96.700, 55.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.870 - 3.000 |
| R-factor | 0.1835 |
| Rwork | 0.173 |
| R-free | 0.25770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.870 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 4698 | |
| Completeness [%] | 99.2 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 25% PEG 1500, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
