4E8X

Lambda-[Ru(TAP)2(dppz-(Me)2)]2+ bound to d(CCGGCGCCGG)2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	47.090, 47.090, 34.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.300 - 2.180
R-factor	0.23521
Rwork	0.233
R-free	0.28370
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	2.694
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.090	2.240
High resolution limit [Å]	2.180	2.180
Number of reflections	2189
Completeness [%]	98.6	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	1ul 2mM DNA, 1ul 2mM lambda-[Ru(TAP)2(dppz-(Me)2)]Cl2, 6ul 10% 2-methyl-2,4-pentanediol, 40mM sodium cacodylate, 12mM spermine tetra-HCl, 80mM potassium chloride, 20mM barium chloride, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K