4E8U
Crystal structure of Arabidopsis IDN2 XS domain along with a small segment of adjacent coiled-coil region
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 52.370, 207.022, 119.629 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.888 - 2.701 |
R-factor | 0.254 |
Rwork | 0.251 |
R-free | 0.30580 |
Structure solution method | SAD (using a smaller construct) and MR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.175 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AutoSol |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
Rmerge | 0.087 | 0.045 | 0.547 |
Number of reflections | 18040 | ||
<I/σ(I)> | 11.2 | ||
Completeness [%] | 99.0 | 97.3 | 96.3 |
Redundancy | 10.3 | 9.6 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M BIS-TRIS pH 6.5, 2.0 M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |