4E8M
Structure of Oceanobacillus iheyensis group II intron in a ligand-free state in the presence of K+ and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.170, 95.160, 224.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.450 - 3.500 |
R-factor | 0.22794 |
Rwork | 0.225 |
R-free | 0.28020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3igi |
RMSD bond length | 0.008 |
RMSD bond angle | 2.472 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.454 | 3.590 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.120 | 1.560 |
Number of reflections | 22159 | |
<I/σ(I)> | 10.1 | 1.5 |
Completeness [%] | 97.6 | 95.6 |
Redundancy | 3.4 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 303 | 100 mM magnesium acetate, 200 mM potassium chloride, 50 mM lithium chloride, 50 mM HEPES sodium, pH 7.0, 4% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 303K |