4E8H
Structural of Bombyx mori glutathione transferase BmGSTD1 complex with GTT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.94968 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.109, 90.818, 87.015 |
Unit cell angles | 90.00, 102.77, 90.00 |
Refinement procedure
Resolution | 47.480 - 2.120 |
R-factor | 0.19638 |
Rwork | 0.195 |
R-free | 0.23075 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e8e |
RMSD bond length | 0.006 |
RMSD bond angle | 1.039 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.190 |
High resolution limit [Å] | 2.110 | 2.110 |
Rmerge | 0.149 | 0.300 |
Number of reflections | 48736 | |
<I/σ(I)> | 13.3 | 4.6 |
Completeness [%] | 95.5 | 95.5 |
Redundancy | 6.4 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 289 | 8% pEG 8000, 0.1M sodium acetate trihydrate (pH 4.6), VAPOR DIFFUSION, HANGING DROP, temperature 289K |