4E8F
Structural Basis for the Activity of a Cytoplasmic RNA Terminal U-transferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9173 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.150, 101.210, 113.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.790 - 2.600 |
| R-factor | 0.1942 |
| Rwork | 0.192 |
| R-free | 0.23880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.970 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 56.800 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 29430 |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 15% (v/v) glycerol, 25.5% (w/v) PEG4000, 170mM ammonium acetate, 85mM tri-sodium citrate, pH 5.6 with 1% taurine, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
