4E80
Structural Basis for the Activity of a Cytoplasmic RNA Terminal U-transferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.973 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.690, 77.960, 151.680 |
| Unit cell angles | 90.00, 109.29, 90.00 |
Refinement procedure
| Resolution | 28.720 - 3.020 |
| R-factor | 0.1777 |
| Rwork | 0.176 |
| R-free | 0.20110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 77.720 |
| High resolution limit [Å] | 3.020 |
| Number of reflections | 35591 |
| Completeness [%] | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 15% (v/v) glycerol, 25.5% (w/v) PEG4000, 170mM ammonium acetate, 85mM tri-sodium citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
