4E7S
Myosin VI D23R I24R R569E (MD) pre-powerstroke state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0716 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.947, 135.140, 196.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.767 - 2.250 |
| R-factor | 0.1813 |
| Rwork | 0.179 |
| R-free | 0.21650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.215 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.300 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.092 | |
| Number of reflections | 102983 | |
| Completeness [%] | 98.8 | 98 |
| Redundancy | 4.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.75 | 291 | 6.75% PEG 8000, 50mM MES, 1mM TCEP, 6% Glycerol, and a stock solution of the protein at 10 mg.ml-1 trapped in the pre-powerstroke state with 2mM Mg.ADP and VO4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
