4E7L
PFV integrase Strand Transfer Complex (STC-Mn*) following reaction in crystallo, at 3.0 A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9763 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 159.078, 159.078, 126.814 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.947 - 3.000 |
R-factor | 0.2299 |
Rwork | 0.228 |
R-free | 0.26430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3os0 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.277 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.099 | 0.524 |
Number of reflections | 32641 | |
<I/σ(I)> | 9.6 | 2.1 |
Completeness [%] | 98.8 | 98.2 |
Redundancy | 3.8 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 291 | 34% PEG-400, 180MM LI2SO4,2.5MM EDTA, 100MM TRIS-HCL, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |