4E71
Crystal structure of the RHO GTPASE binding domain of Plexin B2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97934 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 48.380, 48.380, 157.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.740 - 2.260 |
R-factor | 0.2459 |
Rwork | 0.244 |
R-free | 0.28530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2r2o |
RMSD bond length | 0.010 |
RMSD bond angle | 1.120 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.300 |
High resolution limit [Å] | 2.260 | 6.130 | 2.260 |
Rmerge | 0.098 | 0.064 | 0.999 |
Number of reflections | 5712 | ||
<I/σ(I)> | 8.8 | ||
Completeness [%] | 99.7 | 96.1 | 100 |
Redundancy | 19.6 | 14.2 | 21 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.6 | 291 | 2.5M sodium formate, 0.1M glycine, pH 9.6, vapor diffusion, sitting drop, temperature 291K |