4E6U
Structure of LpxA from Acinetobacter baumannii at 1.4A resolution (P63 form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-06 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 63 |
| Unit cell lengths | 75.751, 75.751, 119.063 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.090 - 1.410 |
| R-factor | 0.17591 |
| Rwork | 0.175 |
| R-free | 0.19147 |
| Structure solution method | 4E6T |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.291 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.460 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.064 | 0.591 |
| Number of reflections | 73881 | |
| <I/σ(I)> | 36.1 | 5 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 11.3 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2ul of 25mg/ml protein, 2uL of 1.8M LiSO4, 0.1M Hepes 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
