4E6F

Crystal structure of a DUF4468 family protein (BACOVA_04320) from Bacteroides ovatus ATCC 8483 at 1.49 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97972,0.91837,0.97917
Spacegroup name	P 1 21 1
Unit cell lengths	49.237, 89.078, 52.586
Unit cell angles	90.00, 105.49, 90.00

Refinement procedure

Resolution	47.449 - 1.490
R-factor	0.1614
Rwork	0.160
R-free	0.18000
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	XSCALE (December 29, 2011)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.449	47.449	1.540
High resolution limit [Å]	1.490	3.210	1.490
Rmerge	0.054	0.028	0.546
Number of reflections	69985	7051	6594
<I/σ(I)>	12.94	35	2.19
Completeness [%]	98.1	97	98
Redundancy	3.42		3.36

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.20M potassium nitrate, 20.00% polyethylene glycol 3350, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K