4E67
The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1) in complex with hydrocinnamoyl-derivatized PLHSpTA peptide
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-15 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.492, 50.906, 57.910 |
| Unit cell angles | 90.00, 101.01, 90.00 |
Refinement procedure
| Resolution | 56.840 - 2.100 |
| R-factor | 0.21801 |
| Rwork | 0.216 |
| R-free | 0.26494 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p2z |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.034 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.840 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 11821 | |
| Completeness [%] | 98.6 | 90.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES (pH 7.5), 0.1 M sodium azide and 22% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
