4E4J

Crystal structure of arginine deiminase from Mycoplasma penetrans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.87260
Spacegroup name	P 1 21 1
Unit cell lengths	120.548, 128.875, 220.279
Unit cell angles	90.00, 91.41, 90.00

Refinement procedure

Resolution	30.000 - 2.300
Rwork	0.210
R-free	0.23850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.325
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.300
Number of reflections	290893
<I/σ(I)>	9.8
Completeness [%]	97.6
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7		1.7 M Ammonium sulfate, pH 7 bis-tris 0.1M amb 15% glyOH, VAPOR DIFFUSION, HANGING DROP