4E42
Structural basis for the recognition of mutant self by a tumor-specific, MHC class II-restricted T cell receptor G4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 168.070, 75.365, 130.859 |
Unit cell angles | 90.00, 123.48, 90.00 |
Refinement procedure
Resolution | 44.630 - 2.700 |
R-factor | 0.20347 |
Rwork | 0.200 |
R-free | 0.26263 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e41 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.437 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.081 | 0.122 |
Number of reflections | 37490 | |
<I/σ(I)> | 25.8 | 17.8 |
Completeness [%] | 99.0 | 99 |
Redundancy | 6.9 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 298 | 1.5 M sodium nitrate 0.1 M sodium citrate, pH 5.0, VAPOR DIFFUSION, temperature 298K |