4E2S
Crystal structure of (S)-Ureidoglycine Aminohydrolase from Arabidopsis thaliana in complex with its substrate, (S)-Ureidoglycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.23985 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.098, 174.894, 154.300 |
| Unit cell angles | 90.00, 99.26, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.590 |
| Rwork | 0.209 |
| R-free | 0.27080 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.21) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.590 |
| Number of reflections | 140969 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M HEPES pH 7.5, 8% (w/v) PEG 8000, 8% (v/v) ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
