4E2Q
Crystal Structure of (S)-Ureidoglycine Aminohydrolase from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97973 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.856, 175.773, 155.176 |
| Unit cell angles | 90.00, 99.39, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| Rwork | 0.224 |
| R-free | 0.28600 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.490 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (AUTOMR) |
| Refinement software | CNS (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.564 | |
| Number of reflections | 137345 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 94.6 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.1M HEPES, 7% (w/v) PEG 8000, 8% (w/v) ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
