4E2K
The structure of the S. aureus DnaG RNA Polymerase Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.115 |
| Spacegroup name | P 61 |
| Unit cell lengths | 151.334, 151.334, 38.571 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.433 - 2.150 |
| R-factor | 0.1921 |
| Rwork | 0.191 |
| R-free | 0.21860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dde |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.804 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 30.430 | 2.220 |
| High resolution limit [Å] | 2.150 | 4.620 | 2.150 |
| Rmerge | 0.072 | ||
| Number of reflections | 28010 | ||
| <I/σ(I)> | 30.5 | ||
| Completeness [%] | 99.8 | 100 | 99 |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.15M sodium thiocyanate, 0.1M Bis-Tris, 13% PEG3350, 2% Benzamidine, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
