4E2E

Crystal structure of a tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide (YWHAG) from Homo sapiens at 2.25 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-07
Detector	DECTRIS PILATUS 6M
Spacegroup name	I 2 2 2
Unit cell lengths	60.988, 78.515, 122.131
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.685 - 2.250
R-factor	0.1912
Rwork	0.189
R-free	0.22930
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	XSCALE (December 6, 2010)
Phasing software	SOLVE
Refinement software	BUSTER-TNT (2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.685	27.685	2.330
High resolution limit [Å]	2.250	4.830	2.250
Rmerge	0.061	0.030	0.904
Number of reflections	14183	2684	2613
<I/σ(I)>	11.83	29.3	1.5
Completeness [%]	98.6	98.3	98
Redundancy	6.08

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	1.00M LiCl, 20.00% PEG-6000, 0.1M HEPES pH 7.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K