4E26

BRAF in complex with an organic inhibitor 7898734

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Detector technology	CCD
Collection date	2007-11-01
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 41 21 2
Unit cell lengths	94.277, 94.277, 163.472
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.550
R-factor	0.22
Rwork	0.220
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.550
Number of reflections	24752
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	298	200 MM Magnesium acetate tetrahydrate, 100 mm sodium cacodylate trihydrate (pH 6.5), and 20% polyethylene glycol 8000, MICROBATCH, temperature 298K