4E1U
[Ru(bpy)2 dppz]2+ bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | .7749 |
| Spacegroup name | P 1 |
| Unit cell lengths | 24.039, 24.797, 37.521 |
| Unit cell angles | 74.67, 84.42, 76.21 |
Refinement procedure
| Resolution | 23.330 - 0.920 |
| R-factor | 0.1401 |
| Rwork | 0.140 |
| R-free | 0.14900 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.620 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.000 |
| High resolution limit [Å] | 0.920 |
| Rmerge | 0.030 |
| Number of reflections | 46920 |
| <I/σ(I)> | 20 |
| Completeness [%] | 82.7 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 296 | 20mM Cacodylate; 6mM Spermine*HCl; 40mM NaCl; 10mM BaCl2 5% 2-M-2,4PD, pH 7.0, VAPOR DIFFUSION, temperature 296K |
