4E0R

Structure of the chicken MHC class I molecule BF2*0401

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-07-06
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	166.196, 40.286, 131.585
Unit cell angles	90.00, 119.71, 90.00

Refinement procedure

Resolution	43.830 - 2.260
R-factor	0.2
Rwork	0.197
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bev
RMSD bond length	0.003
RMSD bond angle	0.771
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX ((PHENIX.REFINE: 1.5_2))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.360
High resolution limit [Å]	2.259	2.260
Rmerge	0.055	0.196
Number of reflections	34950
<I/σ(I)>	19.6	6.8
Completeness [%]	96.6	94
Redundancy	3.1	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.1M HEPES BUFFER (PH 7.0), 5% MPD, 20% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 291K