4E0I
Crystal structure of the C30S/C133S mutant of Erv1 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-01 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 63.280, 77.680, 116.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.530 - 3.000 |
| R-factor | 0.2578 |
| Rwork | 0.255 |
| R-free | 0.30410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e0h |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.902 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.060 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 11797 | |
| Completeness [%] | 99.2 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 15% polyethylene glycol 6000, 0.1M HEPES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
