4E08
Crystal structure of Drosophila melanogaster DJ-1beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-07-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 52.520, 52.520, 227.173 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.720 - 2.000 |
| R-factor | 0.19579 |
| Rwork | 0.193 |
| R-free | 0.24559 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2or3 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.503 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 25116 | |
| <I/σ(I)> | 21.1 | 1.8 |
| Completeness [%] | 97.3 | 83.9 |
| Redundancy | 8.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | 25% polyethylene glycol 4000, 0.2 M ammonium sulfate, 0.1 M sodium acetate pH=4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
