4E04
RpBphP2 chromophore-binding domain crystallized by homologue-directed mutagenesis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-08-03 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.350, 79.860, 149.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.790 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.359 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.800 | 1.840 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Number of reflections | 59311 | |
| <I/σ(I)> | 24.9 | 2.3 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 18% PEG3350, 0.2 mM MgCl2 or 0.2 mM CaCl2 and 0.1 Tris-HCl pH 8.5, VAPOR DIFFUSION, SITTING DROP |
