4DZL
A de novo designed Coiled Coil CC-Tri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.7 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 102.761, 108.441, 41.608 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.420 - 2.300 |
| R-factor | 0.24998 |
| Rwork | 0.248 |
| R-free | 0.28759 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.021 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 21306 | |
| Completeness [%] | 99.6 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M C4H4KNaO6, 20 % w/v PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
